Escalate to service: persistent wavelength errors or high stray light often indicate alignment or optical component faults requiring manufacturer or accredited service. Keep a calibration log documenting dates, standards used, measured values, and corrective actions. The. A spectrophotometer is an analytical instrument that measures how much light a substance absorbs or transmits at specific wavelengths. Handle Cuvettes with Care: Cuvettes are precision optical components. Start by checking your calibration status. If your UV reading is drifting or results are inconsistent across runs, it's time to recalibrate using certified standards.